Journal: Brain Pathology

Article Title: Galectin‐1 Is Implicated in the Protein Kinase C ε/Vimentin‐Controlled Trafficking of Integrin‐β1 in Glioblastoma Cells

doi: 10.1111/j.1750-3639.2008.00227.x

Figure Lengend Snippet: Transient transfection of galectin‐1 using targeted small interfering RNA (siRNA) decreases intracellular galectin‐1 protein. WB analysis of galectin‐1 expression levels at days 5, 7 and 9 post‐transfection in control (ctrl), scramble‐transfected (scr), and galectin‐1‐transfected (siGal1) U87 ( A ) and Hs683 ( C ) cells. Immunofluorescence (IF) analysis of galectin‐1 expression levels in U87 day 7 post‐transfection ( B ) and Hs683 day 5 post‐transfection ( D ) using scrambled or anti‐galectin‐1 siRNA. Top rows are the corresponding bright fields to the IF images.

Article Snippet: The Hs683 [American Type Culture Collection (ATCC) code HTB‐138], U87 (ATCC code HTB‐14), U373 (ATCC code HTB‐17) and T98G (ATCC code CRL‐1690) human GBM cell lines were obtained from the ATCC (Manassas, VA, USA) and maintained in our laboratory as detailed previously , , , .

Techniques: Transfection, Small Interfering RNA, Expressing, Control, Immunofluorescence

Journal: Brain Pathology

Article Title: Galectin‐1 Is Implicated in the Protein Kinase C ε/Vimentin‐Controlled Trafficking of Integrin‐β1 in Glioblastoma Cells

doi: 10.1111/j.1750-3639.2008.00227.x

Figure Lengend Snippet: Integrin‐α9 versus integrin‐β1 expression . A. Integrin‐α9, integrin‐β1 and actin expression across eight glioma cell lines by reverse transcription‐polymerase chain reaction (RT‐PCR) analysis. B. Quantitative RT‐PCR analysis of integrin‐α9 versus integrin‐β1 expression in glioma cell lines Hs683, U87 and U373. C. Hs683 expression levels of integrin‐α9 and integrin‐β1 under control (ctrl), scramble‐transfected (scr) or galectin‐1 siRNA‐transfected (siGal1) conditions obtained through microarray analysis using the Affymetrix Human Genome U133 set Plus 2.0.

Article Snippet: The Hs683 [American Type Culture Collection (ATCC) code HTB‐138], U87 (ATCC code HTB‐14), U373 (ATCC code HTB‐17) and T98G (ATCC code CRL‐1690) human GBM cell lines were obtained from the ATCC (Manassas, VA, USA) and maintained in our laboratory as detailed previously , , , .

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Control, Transfection, Microarray

Journal: Brain Pathology

Article Title: Galectin‐1 Is Implicated in the Protein Kinase C ε/Vimentin‐Controlled Trafficking of Integrin‐β1 in Glioblastoma Cells

doi: 10.1111/j.1750-3639.2008.00227.x

Figure Lengend Snippet: Galectin‐1‐targeted siRNA induces both a decrease of integrin‐β1 at the edges of the cell membrane and an increase in the intracellular accumulation of integrin‐β1 . A. U87 control wild‐type cells ( Aa,Ab ) and U87 cells containing stably transfected antisense galectin‐1 vectors ( Ac ) were co‐stained under non‐permeabilized conditions for integrin‐β1 [the red staining at the tip of actin stress fibers (in green fluorescence)], fibrillar actin (green) and globular actin (red staining inside the cells). Ab. Higher magnification of the control wild‐type ( Aa ) image. B. U87 control wild‐type cells ( Ba ) and U87 cells containing stably transfected antisense galectin‐1 vectors ( Bb ) were stained under permeabilized conditions for integrin‐β1 only (in green), without any staining to reveal actin ( Ba,Bb ). C. U87 and Hs683 cells were transiently transfected with either scrambled siRNA (scr) or siRNA targeted against galectin‐1 (siGal1). Cells were stained for integrin‐β1 (in red) under both permeablized and non‐permeabilized conditions on either day 5 post‐transfection (Hs683) or day 7 post‐transfection (U87).

Article Snippet: The Hs683 [American Type Culture Collection (ATCC) code HTB‐138], U87 (ATCC code HTB‐14), U373 (ATCC code HTB‐17) and T98G (ATCC code CRL‐1690) human GBM cell lines were obtained from the ATCC (Manassas, VA, USA) and maintained in our laboratory as detailed previously , , , .

Techniques: Membrane, Control, Stable Transfection, Transfection, Staining, Fluorescence

Journal: Brain Pathology

Article Title: Galectin‐1 Is Implicated in the Protein Kinase C ε/Vimentin‐Controlled Trafficking of Integrin‐β1 in Glioblastoma Cells

doi: 10.1111/j.1750-3639.2008.00227.x

Figure Lengend Snippet: Western blot (WB) and immunofluorescence (IF) analysis of vimentin and protein kinase C epsilon (PKCε) protein expression levels; galectin‐1‐targeted siRNA increases only vimentin protein levels in Hs683, induces increased perinulear amassment of both PKCε and vimentin, and diminishes the diffuse cytoplasmic staining of PKCε . WB analysis of PKCε and vimentin protein expression levels in U87 ( A ) or Hs683 ( B ) cells untreated (ctrl), scramble‐transfected (scr) or galectin‐1 siRNA‐transfected (siGal1) at days 5, 7 and 9 post‐transfection. IF analysis of PKCε localization in U87 ( C ) or Hs683 ( D ) scr or siGal1 at either day 5 (Hs683) or day 7 (U87) post‐transfection. IF analysis of vimentin localization in U87 ( E ) or Hs683 ( F ) cells scr or galectin‐1 siRNA‐transfected (siGal1) at either day 5 (Hs683) or day 7 (U87) post‐transfection. In all IF figures right columns represent the corresponding bright field images.

Article Snippet: The Hs683 [American Type Culture Collection (ATCC) code HTB‐138], U87 (ATCC code HTB‐14), U373 (ATCC code HTB‐17) and T98G (ATCC code CRL‐1690) human GBM cell lines were obtained from the ATCC (Manassas, VA, USA) and maintained in our laboratory as detailed previously , , , .

Techniques: Western Blot, Immunofluorescence, Expressing, Staining, Transfection

Journal: Brain Pathology

Article Title: Galectin‐1 Is Implicated in the Protein Kinase C ε/Vimentin‐Controlled Trafficking of Integrin‐β1 in Glioblastoma Cells

doi: 10.1111/j.1750-3639.2008.00227.x

Figure Lengend Snippet: Integrin‐α9 versus integrin‐β1 expression . A. Integrin‐α9, integrin‐β1 and actin expression across eight glioma cell lines by reverse transcription‐polymerase chain reaction (RT‐PCR) analysis. B. Quantitative RT‐PCR analysis of integrin‐α9 versus integrin‐β1 expression in glioma cell lines Hs683, U87 and U373. C. Hs683 expression levels of integrin‐α9 and integrin‐β1 under control (ctrl), scramble‐transfected (scr) or galectin‐1 siRNA‐transfected (siGal1) conditions obtained through microarray analysis using the Affymetrix Human Genome U133 set Plus 2.0.

Article Snippet: The Hs683 [American Type Culture Collection (ATCC) code HTB‐138], U87 (ATCC code HTB‐14), U373 (ATCC code HTB‐17) and T98G (ATCC code CRL‐1690) human GBM cell lines were obtained from the ATCC (Manassas, VA, USA) and maintained in our laboratory as detailed previously , , , .

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Control, Transfection, Microarray

Journal: Translational Cancer Research

Article Title: Functional analysis of long-chain non-coding RNA in oral squamous cell carcinoma

doi: 10.21037/tcr.2019.12.67

Figure Lengend Snippet: Clustered panels of differentially expressed lncRNAs (A) and mRNAs (B). Each column represents a sample; each row represents the degree of expression of a gene in different samples, the red refers to relatively high expression, and the green refers to the relatively low expression; the color in the upper left bar represents the fold change of gene expression; the top sample tree represents the clustering of similarity; the top color block represents the expected grouping of samples before the clustering analysis, and the samples with the same color are expected to be in the same group. LncRNA, long-chain non-coding RNA.

Article Snippet: The differentially expressed lncRNA and mRNAs were examined in 5 pairs of tissues with the 4×180 K lncRNA + mRNA Human Gene Expression Microarray V4.0 (CapitalBio Technology, Beijing, China).

Techniques: Expressing, Gene Expression, Blocking Assay

Journal: Translational Cancer Research

Article Title: Functional analysis of long-chain non-coding RNA in oral squamous cell carcinoma

doi: 10.21037/tcr.2019.12.67

Figure Lengend Snippet: Scatter plot of mRNA (A) and lncRNA (B). The number of genes with up-regulated and down-regulated expression was described in the upper left and lower right corners, respectively. The red line X2 is the threshold line of the up-regulated expression, the green line X(−2) is the threshold line of the down-regulated expression, and the middle gray line is the fitted line of the overall expression. The equations in the figure are the fitted line equations, and R represents the correlation coefficient. LncRNA, long-chain non-coding RNA.

Article Snippet: The differentially expressed lncRNA and mRNAs were examined in 5 pairs of tissues with the 4×180 K lncRNA + mRNA Human Gene Expression Microarray V4.0 (CapitalBio Technology, Beijing, China).

Techniques: Expressing

Journal: Translational Cancer Research

Article Title: Functional analysis of long-chain non-coding RNA in oral squamous cell carcinoma

doi: 10.21037/tcr.2019.12.67

Figure Lengend Snippet: Volcano plot of mRNA (A) and lncRNA (B). The horizontal ordinate is the log2 (P values) and the longitudinal ordinate is the log2 (fold change). The red refers to the up-regulated genes, the green refers to the down-regulated genes, and the black refers to genes without significant difference in the expression. Volcanoplot can intuitively reflect the number, significance, and reliability of differentially expressed genes. The closer to the right upper or left upper corner, the more significant the difference in the expression is between two groups. LncRNA, long-chain non-coding RNA.

Article Snippet: The differentially expressed lncRNA and mRNAs were examined in 5 pairs of tissues with the 4×180 K lncRNA + mRNA Human Gene Expression Microarray V4.0 (CapitalBio Technology, Beijing, China).

Techniques: Expressing

Journal: Translational Cancer Research

Article Title: Functional analysis of long-chain non-coding RNA in oral squamous cell carcinoma

doi: 10.21037/tcr.2019.12.67

Figure Lengend Snippet: Disease enrichment analysis of differentially expressed mRNA (top 30 terms). As shown in the figure, the diseases with the most enrichment of mRNAs included immune system diseases, primary immunodeficiency, muscular diseases, allergies and autoimmune diseases, skin and soft tissue diseases, skin diseases, musculoskeletal diseases, celiac diseases, cardiac diseases, inflammatory bowel disease, dilated cardiomyopathy, Graves’ disease, cardiovascular diseases, systemic sclerosis, systemic lupus erythematosus, vitiligo and rheumatoid arthritis.

Article Snippet: The differentially expressed lncRNA and mRNAs were examined in 5 pairs of tissues with the 4×180 K lncRNA + mRNA Human Gene Expression Microarray V4.0 (CapitalBio Technology, Beijing, China).

Techniques: